Journal: International journal of molecular sciences

Article Title: Role of Acyl-CoA Thioesterase 7 in Regulating Fatty Acid Metabolism and Its Contribution to the Onset and Progression of Bovine Clinical Mastitis.

doi: 10.3390/ijms252313046

Figure Lengend Snippet: Figure 4. Distribution analysis of ACOT7 in mammary glands of the two groups. (A) Pathological variation in bovine mammary glands of the healthy control group (Con/C; A1) and clinical mastitis groups (CM; A2) based on H&E staining. (B) ACOT7 distribution in bovine mammary glands of the Con/C (B1) and CM (B2) groups based on immunohistochemical staining. (C) Negative control (NC) for Con/C (C1) and CM (C2) groups. (D) Gray values of positive expression of ACOT7 protein quantified using ImageJ 1.44p software. Con/C, control group. CM, clinical mastitis group. NC, negative control; MA: mammary alveoli; MECs, mammary epithelial cells; NEUT, neutrophil. Scale bars, 50 µm (200× magnification). ** represents p < 0.01.

Article Snippet: The slices were incubated with a rabbit anti-ACOT7 primary antibody at a 1:200 dilution (Proteintech, Wuhan, China).

Techniques: Control, Staining, Immunohistochemical staining, Negative Control, Expressing, Software

Journal: International journal of molecular sciences

Article Title: Role of Acyl-CoA Thioesterase 7 in Regulating Fatty Acid Metabolism and Its Contribution to the Onset and Progression of Bovine Clinical Mastitis.

doi: 10.3390/ijms252313046

Figure Lengend Snippet: Figure 5. Co-localization analysis of ACOT7 in mammary glands of the two groups. (A–D) Co- localization analysis of ACOT7 in bovine mammary glands of the Con/C and CM groups: nuclei (blue, A1,A2), CK-18 (red, B1,B2), ACOT7 (green, C1,C2), merged with CK18 and ACOT7 (D1,D2) staining, respectively. (E) Positive IF signals of ACOT7 protein quantified using ImageJ software. Con/C, control group. CM, clinical mastitis group. MA, mammary alveoli; MECs, mammary epithelial cells; Scale bars, 50 µm (200× magnification). ** represents p < 0.01.

Article Snippet: The slices were incubated with a rabbit anti-ACOT7 primary antibody at a 1:200 dilution (Proteintech, Wuhan, China).

Techniques: Staining, Software, Control

Journal: International journal of molecular sciences

Article Title: Role of Acyl-CoA Thioesterase 7 in Regulating Fatty Acid Metabolism and Its Contribution to the Onset and Progression of Bovine Clinical Mastitis.

doi: 10.3390/ijms252313046

Figure Lengend Snippet: Figure 6. Expression patterns of ACOT7 mRNA and protein in mammary glands of the two groups. (A) Relative expression level of ACOT7 mRNA, monitored via qRT-PCR assays. (B) Protein bands and relative expression level of ACOT7, monitored via Western blot assays and using ImageJ software, respectively. Con/C, control group. CM, clinical mastitis group. Data are presented as means ± SEM. ** represents p < 0.01.

Article Snippet: The slices were incubated with a rabbit anti-ACOT7 primary antibody at a 1:200 dilution (Proteintech, Wuhan, China).

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Software, Control

Journal: International journal of molecular sciences

Article Title: Role of Acyl-CoA Thioesterase 7 in Regulating Fatty Acid Metabolism and Its Contribution to the Onset and Progression of Bovine Clinical Mastitis.

doi: 10.3390/ijms252313046

Figure Lengend Snippet: Figure 7. Proposed molecular mechanism underlying the effects of ACOT7 in the mammary glands of dairy cows with clinical mastitis (CM). MECs, mammary epithelial cells; FABP, fatty-acid-binding protein; FATP, fatty acid transport protein; TLR4, Toll-like receptor 4; TNF-α, tumor necrosis factor-α; IL-1β, interleukin-1β; LPS, lipopolysaccharide. Scale bar, 50 µm (200× magnification).

Article Snippet: The slices were incubated with a rabbit anti-ACOT7 primary antibody at a 1:200 dilution (Proteintech, Wuhan, China).

Techniques: Binding Assay